Mouse Coagulation Factor XII enzyme-linked immunoassay kit
Catalog# EM0041
Mouse Coagulation Factor XII enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
13.72~10000 pg/ml
General Overview
Product Name
Mouse Coagulation Factor XII enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.35 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0164 | 0.0142 | 0.0153 | |
| 13.72 | 0.0253 | 0.0224 | 0.0239 | 0.0086 |
| 41.15 | 0.0286 | 0.0276 | 0.0281 | 0.0128 |
| 123.46 | 0.0472 | 0.0489 | 0.0481 | 0.0328 |
| 370.37 | 0.1131 | 0.1289 | 0.1210 | 0.1057 |
| 1111.11 | 0.3938 | 0.4223 | 0.4081 | 0.3928 |
| 3333.33 | 1.3840 | 1.4040 | 1.3940 | 1.3787 |
| 10000.00 | 3.3980 | 3.3740 | 3.3860 | 3.3707 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 186.2 | 1021.9 | 3453.9 | 143.3 | 846.7 | 2856.7 |
| Standard Deviation | 5.7 | 43.2 | 230.7 | 6.7 | 56.7 | 113.3 |
| Coefficient of Variation (%) | 3.0 | 4.2 | 6.7 | 5.5 | 6.6 | 4.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 Coagulation Factor XII，未加小鼠的Coagulation Factor XII血清作为本底，计算回收率。回收率的范围从 93 % 至 106 %，平均回收率为 100 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 13.00-60.31 | 100 | 33.76 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
Function
凝血因子XII（F12）是一种血清糖蛋白，在启动血液凝结，纤维蛋白溶解以及铁蛋白和血管紧张素的产生中起多种作用。