Mouse Coagulation Factor XII enzyme-linked immunoassay kit(one step)
Catalog# EMY0001
Mouse Coagulation Factor XII enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
4.69~300 ng/ml
General Overview
Product Name
Mouse Coagulation Factor XII enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.22 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0060 | 0.0054 | 0.0057 | |
| 4.69 | 0.0766 | 0.0693 | 0.0730 | 0.0673 |
| 9.38 | 0.1558 | 0.1445 | 0.1502 | 0.1445 |
| 18.75 | 0.2989 | 0.3013 | 0.3001 | 0.2944 |
| 37.50 | 0.6302 | 0.5610 | 0.5956 | 0.5899 |
| 75.00 | 1.2021 | 1.2191 | 1.2106 | 1.2049 |
| 150.00 | 2.4639 | 2.3883 | 2.4261 | 2.4204 |
| 300.00 | 4.0653 | 4.0355 | 4.0504 | 4.0447 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.6 | 30.7 | 103.6 | 4.3 | 25.4 | 85.7 |
| Standard Deviation | 0.2 | 1.3 | 6.9 | 0.2 | 1.7 | 3.4 |
| Coefficient of Variation (%) | 3.0 | 4.2 | 6.7 | 5.5 | 6.6 | 4.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 Coagulation Factor XII，未加小鼠 Coagulation Factor XII 的血清作为本底，计算回收率。回收率的范围从 93 % 至 106 %，平均回收率为 100 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 29.16-54.27 | 100.0 | 42.19 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7点
Incubation Count
2
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
凝血因子XII（F12）是一种血清糖蛋白，在启动血液凝结，纤维蛋白溶解以及铁蛋白和血管紧张素的产生中起多种作用。