Mouse Leptin enzyme-linked immunoassay kit
Catalog# EM0027
Mouse Leptin enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Mouse
Standard curve range
-
312.50~20000 pg/ml
General Overview
Product Name
Mouse Leptin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
19.30 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0167 | 0.0168 | 0.0168 | |
| 312.50 | 0.0951 | 0.0962 | 0.0957 | 0.0789 |
| 625.00 | 0.1774 | 0.1735 | 0.1755 | 0.1587 |
| 1250.00 | 0.3333 | 0.3394 | 0.3364 | 0.3196 |
| 2500.00 | 0.6285 | 0.6269 | 0.6277 | 0.6110 |
| 5000.00 | 1.2010 | 1.1910 | 1.1960 | 1.1793 |
| 10000.00 | 2.1700 | 2.1260 | 2.1480 | 2.1313 |
| 20000.00 | 3.5340 | 3.6220 | 3.5780 | 3.5613 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 427.5 | 1982.7 | 5614.0 | 422.2 | 1991.2 | 6030.1 |
| Standard Deviation | 12.3 | 48.3 | 268.1 | 7.7 | 81.3 | 142.6 |
| Coefficient of Variation (%) | 2.9 | 2.4 | 4.8 | 1.8 | 4.1 | 2.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠Leptin，未加小鼠Leptin 的血清作为本底，计算回收率。回收率的范围从 72 % 至 119 %，平均回收率为 97 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2244.18-16867.46 | 100 | 8181.3 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Mouse
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Dosing Volume
50 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
瘦素是小鼠肥胖基因的产物。具有阻断瘦素合成的肥胖基因突变的小鼠肥胖和糖尿病，并且活动，代谢和体温降低。大鼠瘦素分别与小鼠和人类蛋白质共享约96%和82%的序列同一性。瘦素mRNA的表达仅限于脂肪组织。