Mouse TNF-α enzyme-linked immunoassay kit
Catalog# EM0010
Mouse TNF-α enzyme-linked immunoassay kit
-
ELISA
-
Mouse
-
1.37~1000 pg/ml
General Overview
Product Name
Mouse TNF-α enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.097 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0121 | 0.0111 | 0.0116 | |
| 1.37 | 0.0242 | 0.0223 | 0.0233 | 0.0117 |
| 4.12 | 0.0482 | 0.0444 | 0.0463 | 0.0347 |
| 12.35 | 0.1134 | 0.1092 | 0.1113 | 0.0997 |
| 37.04 | 0.3165 | 0.3032 | 0.3099 | 0.2983 |
| 111.11 | 0.8042 | 0.7996 | 0.8019 | 0.7903 |
| 333.33 | 1.9672 | 1.9564 | 1.9618 | 1.9502 |
| 1000 | 4.1683 | 4.2715 | 4.2199 | 4.2083 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.1 | 80.4 | 271.7 | 14 | 75.2 | 236.7 |
| Standard Deviation | 1.1 | 4.8 | 16 | 0.3 | 4 | 10.8 |
| Coefficient of Variation (%) | 6.5 | 5.9 | 5.9 | 2 | 5.3 | 4.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠TNF-α，未加小鼠 TNF-α 的血清作为本底，计算回收率。
回收率的范围从 107 % 至 132 %，平均回收率为 116 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.28 | 33 | 0.16 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Mouse | See 2 publications below |
Target
UNIPROT
Function
Citation
-
An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing
Author: Zhou Ziyi, Zhang Dengjun, Ning Xuchao, Jin Linbo, Lin Yijing, Liang Chen, Wen Xin, Huang Tianhao, Zhou Junli, Zhang Yiming
PMID: 39871272
Journal: Journal Of Nanobiotechnology
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
-
Citation
-
(−)-Epicatechin gallate prevented atherosclerosis by reducing abnormal proliferation of VSMCs and oxidative stress of AML 12 cells
Author: Yu Jinjin,et al
PMID: 38936786
Journal: Cellular Signalling
Application: ELISA
Reactivity: Mouse
Publish date: 2024 Jul
-
Citation