Mouse IL-6 enzyme-linked immunoassay kit
Catalog# EM0004
Mouse IL-6 enzyme-linked immunoassay kit
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ELISA
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Mouse
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1.37~1000 pg/ml
General Overview
Product Name
Mouse IL-6 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.29 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0224 | 0.0228 | 0.0226 | |
| 1.37 | 0.0267 | 0.0253 | 0.026 | 0.0034 |
| 4.12 | 0.0359 | 0.0351 | 0.0355 | 0.0129 |
| 12.35 | 0.0665 | 0.0635 | 0.065 | 0.0424 |
| 37.04 | 0.1438 | 0.1432 | 0.1435 | 0.1209 |
| 111.11 | 0.3781 | 0.3824 | 0.3803 | 0.3577 |
| 333.33 | 1.0371 | 0.9801 | 1.0086 | 0.986 |
| 1000 | 2.7831 | 2.7443 | 2.7637 | 2.7411 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 18.9 | 112.8 | 346.8 | 19.2 | 111.5 | 346.5 |
| Standard Deviation | 0.6 | 3.7 | 10.9 | 0.9 | 5.1 | 8.3 |
| Coefficient of Variation (%) | 3.1 | 3.3 | 3.1 | 4.9 | 4.6 | 2.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠IL-6，未加小鼠 IL-6 的血清作为本底，计算回收率。
回收率的范围从 89 % 至 118 %，平均回收率为 104 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-15.85 | 88 | 2.71 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Mouse | See 2 publications below |
Target
UNIPROT
Function
Citation
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An antibacterial, antioxidant and hemostatic hydrogel accelerates infectious wound healing
Author: Zhou Ziyi, Zhang Dengjun, Ning Xuchao, Jin Linbo, Lin Yijing, Liang Chen, Wen Xin, Huang Tianhao, Zhou Junli, Zhang Yiming
PMID: 39871272
Journal: Journal Of Nanobiotechnology
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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Citation
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Interleukin-17 alleviates erastin-induced alveolar bone loss by suppressing ferroptosis via interaction between NRF2 and p-STAT3
Author: Bao J, Wang Z, Yang Y, et al
PMID: 37961757
Journal: Journal Of Clinical Periodontology
Application: ELISA
Reactivity: Mouse
Publish date: 2023 Nov
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Citation