Mouse IL-10 enzyme-linked immunoassay kit
Catalog# EM0005
Mouse IL-10 enzyme-linked immunoassay kit
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ELISA
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Mouse
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13.72~10000 pg/ml
General Overview
Product Name
Mouse IL-10 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
5.75 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0207 | 0.0209 | 0.0208 | |
| 13.72 | 0.0333 | 0.0311 | 0.0322 | 0.0114 |
| 41.15 | 0.0586 | 0.0553 | 0.0570 | 0.0362 |
| 123.46 | 0.1152 | 0.11215 | 0.1136 | 0.0928 |
| 370.37 | 0.3011 | 0.2984 | 0.2998 | 0.2790 |
| 1111.11 | 0.7795 | 0.7776 | 0.7786 | 0.7578 |
| 3333.33 | 1.9981 | 1.9036 | 1.9505 | 1.9297 |
| 10000.00 | 3.7131 | 3.7112 | 3.7120 | 3.6912 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 179.9 | 784.5 | 2838.4 | 218.4 | 918.7 | 2985.2 |
| Standard Deviation | 6.2 | 34.5 | 140.3 | 11.0 | 58.2 | 166.5 |
| Coefficient of Variation (%) | 3.5 | 4.4 | 4.9 | 5.0 | 6.4 | 6.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 IL-10，未加小鼠IL-10 的血清作为本底，计算回收率。回收率的范围从 84 % 至 119 %，平均回收率为 103 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 19.31-941.79 | 100 | 120.83 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Mouse | See 2 publications below |
Target
UNIPROT
Function
Citation
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Decellularized Extracellular Matrix Scaffold Loaded with Regulatory T Cell-Conditioned Medium Induces M2 Macrophage Polarization
Author: Hongjing Jiang, Xuheng Sun, Jiang Liu, Lijun Fang, Yuanfeng Liang, Jiahui Zhou, Yueheng Wu, Zhanyi Lin
PMID: 40255505
Journal: Biomaterials Research
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Apr
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Citation
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Indoleamine 2,3-dioxygenase 1 alters the proportions of B cell subpopulations in the microenvironment of acute myeloid leukemia
Author: Yao Yu, Liu Yu-ying, Li Jian-feng, Chen Yun-shuo, Shi Lei, Shen Yang, Yang Li-li, Yang Qing
PMID: 40234305
Journal: Molecular Biomedicine
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Apr
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Citation