Mouse GM-CSF/CSF2 enzyme-linked immunoassay kit
Catalog# EM0011
Mouse GM-CSF/CSF2 enzyme-linked immunoassay kit
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ELISA
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Mouse
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Mouse GM-CSF/CSF2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.48 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0110 | 0.0112 | 0.0111 | |
| 1.37 | 0.0157 | 0.0179 | 0.0168 | 0.0057 |
| 4.12 | 0.0294 | 0.0289 | 0.0292 | 0.0181 |
| 12.35 | 0.0728 | 0.0767 | 0.0748 | 0.0637 |
| 37.04 | 0.2205 | 0.2186 | 0.2196 | 0.2085 |
| 111.11 | 0.5898 | 0.6276 | 0.6087 | 0.5976 |
| 333.33 | 1.5610 | 1.5660 | 1.5635 | 1.5524 |
| 1000.00 | 2.8900 | 2.8870 | 2.8885 | 2.8774 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 13.4 | 84.9 | 289.1 | 14.3 | 78.5 | 315.9 |
| Standard Deviation | 0.6 | 2.9 | 13.2 | 0.57 | 2.5 | 14.9 |
| Coefficient of Variation (%) | 4.8 | 3.4 | 4.6 | 3.9 | 3.2 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse GM-CSF/CSF2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 110% with an overall mean recovery of 92%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-7.02 | 50.0 | 1.04 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of GM-CSF/CSF2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Colony stimulating factor 2 (granulocyte-macrophage)
Colony Stimulating Factor 2
Colony stimulating factor
Colony-stimulating factor
CSF 2
CSF
CSF2
CSF2_HUMAN
GM-CSF
GMCSF
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