Mouse VEGF enzyme-linked immunoassay kit
Catalog# EM0013
Mouse VEGF enzyme-linked immunoassay kit
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ELISA
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Mouse
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4.12~3000 pg/ml
Overview
Product Name
Mouse VEGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.57 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0084 | 0.0091 | 0.0088 | |
| 4.12 | 0.0241 | 0.0231 | 0.0236 | 0.0149 |
| 12.35 | 0.0557 | 0.0526 | 0.0542 | 0.0454 |
| 37.04 | 0.1373 | 0.1337 | 0.1355 | 0.1268 |
| 111.11 | 0.3702 | 0.3549 | 0.3626 | 0.3538 |
| 333.33 | 0.9362 | 0.9147 | 0.9255 | 0.9167 |
| 1000.00 | 2.0741 | 2.0273 | 2.0507 | 2.0420 |
| 3000.00 | 3.6762 | 3.6825 | 3.6794 | 3.6706 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 59.4 | 340.6 | 878.4 | 64.3 | 347.1 | 993.3 |
| Standard Deviation | 1.8 | 9.8 | 21.3 | 2.7 | 12.1 | 20.0 |
| Coefficient of Variation (%) | 3.0 | 2.9 | 2.2 | 4.2 | 3.5 | 2.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠 VEGF，未加小鼠 VEGF 的血清作为本底，计算回收率。回收率的范围从 114 % 至 131 %，平均回收率为 122 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 16.98-45.87 | 100 | 27.58 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Mouse | See 1 publications below |
Target
UNIPROT
Function
Citation
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Bruceine A Abrogates Pancreatic Cancer Metastasis by Suppressing PFKFB4-Glycolysis-EMT Axis
Journal: Phytomedicine
DOI: 10.1016/j.phymed.2025.157665
IF: 8.3
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Dec