Mouse IL-4 enzyme-linked immunoassay kit
Catalog# EM0003
Mouse IL-4 enzyme-linked immunoassay kit
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ELISA
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Mouse
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2.74~2000 pg/ml
Overview
Product Name
Mouse IL-4 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.35 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0351 | 0.0332 | 0.0342 | |
| 2.74 | 0.0571 | 0.0641 | 0.0606 | 0.038 |
| 8.23 | 0.0947 | 0.0921 | 0.0934 | 0.0708 |
| 24.69 | 0.1691 | 0.1622 | 0.1657 | 0.1431 |
| 74.07 | 0.3641 | 0.3542 | 0.3592 | 0.3366 |
| 222.22 | 0.8632 | 0.8337 | 0.8485 | 0.8259 |
| 666.67 | 2.1632 | 2.1331 | 2.1482 | 2.1256 |
| 2000 | 3.9181 | 3.9331 | 3.9256 | 3.903 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 25.6 | 135.5 | 374.7 | 21.1 | 102.4 | 438.7 |
| Standard Deviation | 1.1 | 4.7 | 13.8 | 1.0 | 8.4 | 35.2 |
| Coefficient of Variation (%) | 4.5 | 3.5 | 3.7 | 4.9 | 8.2 | 8.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康小鼠血清加入 3 个不同浓度水平的小鼠IL-4，未加小鼠 IL-4 的血清作为本底，计算回收率。回收率的范围从 99 % 至 120 %，平均回收率为 112 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.37 | 4.2 | 0.37 |
应用本试剂盒，检测 30 个健康小鼠的血清样本，小鼠的用药史不详。 n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Mouse | See 2 publications below |
Target
UNIPROT
Function
Citation
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Identification of a dual JAK3/TEC family kinase inhibitor for atopic dermatitis therapy
Journal: Biochemical Pharmacology
DOI:
IF: 5.3
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
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Identification of a dual JAK3/TEC family kinase inhibitor for atopic dermatitis therapy
Journal: BIOCHEMICAL PHARMACOLOGY
DOI: 10.1016/j.bcp.2025.116740
IF: 5.6
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan