Human IL-1β enzyme-linked immunoassay kit

Click for inquiry

Contact Us

Email: sales@huabio.cn

Phone: 0571-88062880

Find local distributors

Catalog# EH0006

Human IL-1β enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

This product has been cited in peer reviewed publications, see list HERE

General Overview

Product Name

Human IL-1β enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.77 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0	0.0243	0.0232	0.0238
1.37	0.0294	0.0277	0.0286	0.0048
4.12	0.0396	0.0382	0.0389	0.0152
12.35	0.078	0.0693	0.0737	0.0499
37.04	0.171	0.1696	0.1703	0.1466
111.11	0.4662	0.4216	0.4439	0.4202
333.33	1.1176	1.0011	1.0594	1.0356
1000	2.4482	2.2666	2.3574	2.3337

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	19.2	97.8	341.4	20.3	107.9	346.9
Standard Deviation	0.5	6.1	23.7	0.7	3.6	13.8
Coefficient of Variation (%)	2.8	6.2	6.9	3.5	3.3	4

板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。

板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。

Spike Recovery

健康人血清加入 3 个不同浓度水平的人IL-1β，未加人 IL-1β 的血清作为本底，计算回收率。

回收率的范围从 113 % 至 137 %，平均回收率为 125 %。

Sample Value

Sample Matrix	Evaluated Sample	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-104.5	78	12.27

应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。

n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7点/3倍

Incubation Count

Dosing Volume

10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

P01584

Function

IL-1 表示两种多效性细胞因子IL-1α（IL-1F1）和IL-1β（IL-1F2）的总称，它们是不同基因的产物。IL-1α 和 IL-1β 是结构相关的多肽，在人类中具有约 21% 的氨基酸（aa）序列同一性。这两种蛋白质都是由多种细胞对炎症因子、感染或微生物内毒素做出反应而产生的。虽然IL-1α和IL-1β是独立调节的，但它们与相同的受体结合并发挥相同的生物学效应。IL-1 RI 直接与 IL-1α 或 IL-1β 结合，然后与 IL-1 R 辅助蛋白（IL-1 R3/IL-1 R AcP）结合，形成能够进行信号转导的高亲和力受体复合物。IL-1 RII对IL-1β具有高亲和力，但作为诱饵受体和IL-1β活性的负调节因子起作用。IL-1ra通过阻止IL-1α和IL-1β与IL-1 RI相互作用来充当竞争性拮抗剂。人类IL-1β cDNA编码269氨基酸前体。116氨基酸前肽被半胱氨酸蛋白酶IL-1β转换酶（半胱天冬酶-1 / ICE）在细胞内裂解以产生活性细胞因子。17 kDa成熟人IL-1β与恒河猴共享96%的氨基酸序列同一性，与犬、棉鼠、马、猫、小鼠、猪和大鼠IL-1β共享67%-78%。

Citation

Filter:

A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment

Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang

PMID: 39735335
Journal: Bioactive Materials
- Application:
- Reactivity:
- Publish date: 2024 Dec
- Citation

Human IL-1β enzyme-linked immunoassay kit

Contact Us

General Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

Citation

Same Target and Pathway Products

Human EGF enzyme-linked immunoassay kit

Human CCL5/RANTES enzyme-linked immunoassay kit

Human CXCL10/IP-10 enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit

Human Granzyme B enzyme-linked immunoassay kit

Human GM-CSF enzyme-linked immunoassay kit

Human Prolactin enzyme-linked immunoassay kit

Human MBL2 enzyme-linked immunoassay kit

Human IL-31 enzyme-linked immunoassay kit

Human NCAM 1/CD56 enzyme-linked immunoassay kit

Human CD200R1 enzyme-linked immunoassay kit

Human CCL2 enzyme-linked immunoassay kit

Human IFN-α enzyme-linked immunoassay kit

Human IL-12B/IL-12 p40 enzyme-linked immunoassay kit

Human HVEM/TNFRSF14 enzyme-linked immunoassay kit

Human CD33 enzyme-linked immunoassay kit

Human CRP enzyme-linked immunoassay kit

Human LIF enzyme-linked immunoassay kit

Human IL-23 p19 enzyme-linked immunoassay kit

Human CD85d enzyme-linked immunoassay kit

Human CCL25 enzyme-linked immunoassay kit

Human CD80 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human cIAP2 enzyme-linked immunoassay kit

Human MICB enzyme-linked immunoassay kit

Human IL-3R alpha/CD123 enzyme-linked immunoassay kit

Human von Willebrand Factor enzyme-linked immunoassay kit（one step）

Human MMP-13 enzyme-linked immunoassay kit

Human Complement C5 enzyme-linked immunoassay kit（one step）

Human BCMA/TNFRSF17 enzyme-linked immunoassay kit

Human Growth Hormone enzyme-linked immunoassay kit

Human Pentraxin 3/PTX3 enzyme-linked immunoassay kit

Human FOLR1 enzyme-linked immunoassay kit

Human IL-5 enzyme-linked immunoassay kit

Human IL-17E enzyme-linked immunoassay kit

Human IL-13 enzyme-linked immunoassay kit

Human PD-L1/B7-H1 enzyme-linked immunoassay kit

Human beta-NGF enzyme-linked immunoassay kit

Human PDGF-AA enzyme-linked immunoassay kit

Human ICAM-1 / CD54 enzyme-linked immunoassay kit

Human CD147/EMMPRIN enzyme-linked immunoassay kit

Human OX40/TNFRSF4 enzyme-linked immunoassay kit

Human VEGF enzyme-linked immunoassay kit

Human MICA enzyme-linked immunoassay kit

Human IL-18 enzyme-linked immunoassay kit

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human IL-18BP enzyme-linked immunoassay kit

Human GITR/TNFRSF18 enzyme-linked immunoassay kit

Human VE Cadherin enzyme-linked immunoassay kit

Human IFN-gamma R1 enzyme-linked immunoassay kit

Human MMP-8 enzyme-linked immunoassay kit

Human Adiponectin enzyme-linked immunoassay kit

Human IL-7 enzyme-linked immunoassay kit

Human CD30 enzyme-linked immunoassay kit

Human TGF-α enzyme-linked immunoassay kit