Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Catalog# EH0024
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
-
ELISA
-
Human
-
2.74~2000 pg/ml
General Overview
Product Name
Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.51 pg/ml (50 μl)；9.93 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0150 | 0.0150 | 0.0150 | |
| 2.74 | 0.0210 | 0.0220 | 0.0215 | 0.0065 |
| 8.23 | 0.0295 | 0.0310 | 0.0303 | 0.0153 |
| 24.69 | 0.0601 | 0.0550 | 0.0576 | 0.0426 |
| 74.07 | 0.1620 | 0.1457 | 0.1539 | 0.1389 |
| 222.22 | 0.5740 | 0.4956 | 0.5348 | 0.5198 |
| 666.67 | 2.2035 | 1.9879 | 2.0957 | 2.0807 |
| 2000.00 | 4.5685 | 4.4910 | 4.5298 | 4.5148 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 36.8 | 179.7 | 464.2 | 31.66 | 170.64 | 581.98 |
| Standard Deviation | 1.4 | 6.9 | 13.8 | 2.38 | 11.59 | 25.24 |
| Coefficient of Variation (%) | 3.9 | 3.8 | 3 | 7.5 | 6.8 | 4.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人FGF，未加人 FGF 的血清作为本底，计算回收率。回收率的范围从 78 % 至 92 %，平均回收率为 86 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.45-24.74 | 100 | 15.01 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Mouse | See 1 publications below |
Target
UNIPROT
Function
Citation
-
Inhalable Chemotactic Liposome for Targeted Modulation of Pulmonary Pre-Metastatic Niche
Author: Ziyan Wang, Longfei Liao, Fengju Wang, Junzhu Shi, Jiaqi Liu, Rui Zhou, Min Liu, Yuan Huang, Lian Li
PMID: 7NOPMID25030501
Journal: Advanced Functional Materials
Application: ELISA
Reactivity: Mouse
Publish date: 2025 Jan
-
Citation
-
A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang
PMID: 39735335
Journal: Bioactive Materials
Application:
Reactivity:
Publish date: 2024 Dec
-
Citation