Human Leptin enzyme-linked immunoassay kit
Catalog# EH0056
Human Leptin enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
40.96~10000 pg/ml
General Overview
Product Name
Human Leptin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
9.45 pg/ml (50 μl)；18.83 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0090 | 0.0090 | 0.0090 | |
| 40.96 | 0.0129 | 0.0136 | 0.0133 | 0.0043 |
| 102.40 | 0.0211 | 0.0208 | 0.0210 | 0.0120 |
| 256.00 | 0.0549 | 0.0544 | 0.0547 | 0.0457 |
| 640.00 | 0.2327 | 0.2207 | 0.2267 | 0.2177 |
| 1600.00 | 1.0060 | 0.9558 | 0.9809 | 0.9719 |
| 4000.00 | 2.7070 | 2.6740 | 2.6905 | 2.6815 |
| 10000.00 | 3.8850 | 3.8240 | 3.8545 | 3.8455 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 220.9 | 1012.4 | 3975.4 | 245.9 | 1135.2 | 4163.7 |
| Standard Deviation | 4.8 | 30.5 | 203.4 | 13.1 | 67.5 | 262.1 |
| Coefficient of Variation (%) | 2.2 | 3.0 | 5.1 | 5.3 | 5.9 | 6.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人Leptin，未加人 Leptin 的血清作为本底，计算回收率。回收率的范围从 89 % 至 118 %，平均回收率为 104 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 393.86-7522.16 | 100 | 4332.2 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
瘦素是小鼠肥胖基因的产物。具有阻断瘦素合成的肥胖基因突变的小鼠是肥胖和糖尿病患者，并且活性，新陈代谢和体温降低。大鼠瘦素分别与小鼠和人类蛋白共享约96%和82%的序列同一性。瘦素mRNA的表达仅限于脂肪组织。