Human IL-2 enzyme-linked immunoassay kit

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Catalog# EH0013

Human IL-2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

6.86~5000 pg/ml

This product has been cited in peer reviewed publications, see list HERE

General Overview

Product Name

Human IL-2 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

1.67 pg/ml (50 μl) ；3.08 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0112	0.0111	0.0112
6.86	0.0212	0.0173	0.0193	0.0085
20.58	0.0301	0.0282	0.0292	0.0175
61.73	0.0671	0.0623	0.0647	0.0530
185.19	0.2142	0.1983	0.2063	0.1955
555.56	0.8721	0.7723	0.8222	0.8105
1666.67	2.4952	2.4073	2.4513	2.4401
5000.00	4.2972	4.3173	4.3073	4.2961

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	92.1	407.5	1540.9	102.1	502.1	1990.9
Standard Deviation	4.7	23.4	86.4	6.0	29.1	119.09
Coefficient of Variation (%)	5.1	5.7	5.6	5.9	5.8	6.0

板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。

板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。

Spike Recovery

健康人血清加入 3 个不同浓度水平的人 IL-2，未加人 IL-2 的血清作为本底，计算回收率。回收率的范围从 85 % 至 115 %，平均回收率为 103 %。

Sample Value

Sample Matrix	Evaluated Sample	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.	0	0

应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。

n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7点/3倍

Incubation Count

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

P60568

Function

白细胞介素2（IL-2），也称为T细胞生长因子（TCGF），是一种15-18 kDa可变糖基化的α-螺旋多肽，是普通γ链（γc）细胞因子家族的成员。它以单体形式存在，半衰期非常短（<30分钟）。人IL-2合成为153个氨基酸（aa）的前体，其中包含20 aa信号序列和133 aa成熟区域。成熟区域本质上是α-螺旋的，在Thr3处包含一个利用的O-连接糖基化位点加上三个半胱氨酸，其中两个形成对活性至关重要的链内二硫键。成熟的人类IL-2与犬，大鼠，猫和恒河猴IL-2分别具有73%，66%，78%和97%的aa身份。尽管人IL-2与高度多态性小鼠IL-2仅共享约60%的aa同一性，但已知人IL-2在小鼠IL-2反应细胞上具有活性。据报道分泌IL-2的细胞包括γ δ T细胞，活化的常规CD4+和CD8 + T细胞，神经元，小胶质细胞和造血干细胞。 IL-2 （IL-2 R）的受体由三个亚基组成，即 55 kDa CD25/IL-2 R α 链、70 kDa IL-2 R β 链和 65 kDa 普通 γ 链。IL-2首先与CD25结合，二元复合物然后募集IL-2 R β和γc形成四元信号复合物。除IL-2外，IL-15在其四元信号复合物中使用IL-2 R beta。γ c也是IL-4，-7，-9，-15和-21的信号受体。体外研究表明IL-2在T细胞活化和扩增中具有重要作用。在体内，IL-2对于调节性T细胞（Treg）的发育，维持和功能至关重要，调节性T细胞提供针对自身免疫性疾病的保护。另一方面，IL-2还可以通过其在调节T细胞运输基因表达和Th2细胞因子产生中的作用来促进靶器官的自身免疫性炎症。在CD8 + T细胞亚群中，IL-2对于最佳原发反应和分化为末端效应细胞至关重要。IL-2还促进活化的CD8 + T细胞发育成记忆细胞。

Citation

Filter:

A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment

Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang

PMID: 39735335
Journal: Bioactive Materials
- Application:
- Reactivity:
- Publish date: 2024 Dec
- Citation

Human IL-2 enzyme-linked immunoassay kit

Contact Us

General Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

Citation

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