Human IL-18 enzyme-linked immunoassay kit
Catalog# EH0053
Human IL-18 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
General Overview
Product Name
Human IL-18 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.56 pg/ml (50 μl)；1.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0449 | 0.0488 | 0.0469 | |
| 6.86 | 0.0752 | 0.0687 | 0.0720 | 0.0251 |
| 20.58 | 0.1038 | 0.0908 | 0.0973 | 0.0505 |
| 61.73 | 0.1749 | 0.1668 | 0.1709 | 0.1240 |
| 185.19 | 0.4421 | 0.4094 | 0.4258 | 0.3789 |
| 555.56 | 1.1370 | 1.1090 | 1.1230 | 1.0762 |
| 1666.67 | 2.7832 | 2.6478 | 2.7155 | 2.6687 |
| 5000.00 | 4.2824 | 4.3490 | 4.3157 | 4.2689 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 99.5 | 490.4 | 1512.9 | 101.2 | 481.1 | 1476.2 |
| Standard Deviation | 5.0 | 32.7 | 74.9 | 3.7 | 22.8 | 86.3 |
| Coefficient of Variation (%) | 5.1 | 6.7 | 4.9 | 3.6 | 4.7 | 5.8 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-18，未加人 IL-18的血清作为本底，计算回收率。回收率的范围从 84 % 至 111 %，平均回收率为 102 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 249.34- 3143.95 | 100 | 1382.96 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Human | See 1 publications below |
Target
UNIPROT
Function
Citation
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A high-throughput reporter assay for screening potential gasdermin D inhibitors from natural products
Author:
PMID: 10.1016/j.ijbiomac.2026.150110
Journal: International Journal Of Biological Macromolecules
Application: ELISA
Reactivity: Human
Publish date: 2026 Jan
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Citation