Human IFN-γ enzyme-linked immunoassay kit

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Catalog# EH0039

Human IFN-γ enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

This product has been cited in peer reviewed publications, see list HERE

General Overview

Product Name

Human IFN-γ enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.36 pg/ml (50 μl)；0.85 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0117	0.0121	0.0119
1.37	0.0250	0.0282	0.0266	0.0147
4.12	0.0462	0.0489	0.0476	0.0357
12.35	0.1248	0.1240	0.1244	0.1125
37.04	0.3399	0.3393	0.3396	0.3277
111.11	0.9201	0.8954	0.9078	0.8959
333.33	2.0160	1.9910	2.0035	1.9916
1000.00	3.6940	3.6730	3.6835	3.6716

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	17.2	98.2	273.3	18.9	91.8	240.1
Standard Deviation	0.5	3	6.8	0.8	2.7	5.2
Coefficient of Variation (%)	2.8	3.2	2.5	4.2	3	2.1

板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。

板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。

Spike Recovery

健康人血清加入 3 个不同浓度水平的人IFN-γ，未加人 IFN-γ 的血清作为本底，计算回收率。

回收率的范围从 89 % 至 113 %，平均回收率为 105 %。

Sample Value

Sample Matrix	Evaluated Sample	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-446.28	92	93.56

应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。

n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

75min

Standard Curve Gradient

7点/3倍

Incubation Count

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

P01579

Function

干扰素-γ（IFN-γ），也称为II型或免疫干扰素，发挥广泛的免疫调节活性，被认为是原型促炎细胞因子。成熟的人的IFN-γ以20-25kDa可变糖基化亚基的非共价连接的同源二聚体存在。它与恒河猴IFN-γ共享90%的氨基酸（aa）序列同一性，与牛、犬、马、猫和猪IFN-γ共享59%-64%氨基酸序列同一性，与棉花大鼠、小鼠和大鼠IFN-γ共享37%-43%氨基酸序列同一性。IFN-γ二聚体与IFN-γ RI（α亚基）结合，然后与IFN-γ-RII（β亚基）相互作用，形成两个α和两个β亚基的功能受体复合物。IFN-γ RII的加入增加了配体的结合亲和力和信号转导的效率。IFN-γ由多种免疫细胞在炎症条件下产生，特别是由T细胞和NK细胞产生。它通过促进Th1细胞的发育和活化，单核细胞和巨噬细胞的化学吸引和活化，抗原呈递分子的上调以及B细胞中的免疫球蛋白类别转换，在宿主防御中起关键作用。它还表现出抗病毒、抗增殖和凋亡作用。此外，IFN-γ通过促进调节性T细胞的发育和抑制Th17细胞分化来发挥抗炎介质的作用。IFN-γ的多效性作用有助于动脉粥样硬化的多个方面的发展。

Citation

Filter:

A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment

Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang

PMID: 39735335
Journal: Bioactive Materials
- Application:
- Reactivity:
- Publish date: 2024 Dec
- Citation

Human IFN-γ enzyme-linked immunoassay kit

Contact Us

General Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

Citation

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