Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
Catalog# EH0028
Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
10.97~8000 pg/ml
General Overview
Product Name
Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
8.58 pg/ml (50 μl)；12.19 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0175 | 0.0195 | 0.0185 | |
| 10.97 | 0.0264 | 0.0238 | 0.0251 | 0.0066 |
| 32.92 | 0.0320 | 0.0311 | 0.0316 | 0.0131 |
| 98.77 | 0.0640 | 0.0629 | 0.0635 | 0.0450 |
| 296.30 | 0.2220 | 0.2240 | 0.2230 | 0.2045 |
| 888.89 | 0.9390 | 0.9138 | 0.9264 | 0.9079 |
| 2666.67 | 2.4702 | 2.4450 | 2.4576 | 2.4391 |
| 8000.00 | 3.9750 | 4.0220 | 3.9985 | 3.9800 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 78.1 | 395.8 | 1027.1 | 82.4 | 379.2 | 1127.4 |
| Standard Deviation | 2.4 | 14.8 | 30.9 | 4.2 | 24.1 | 74.6 |
| Coefficient of Variation (%) | 3.1 | 3.7 | 3.0 | 4.0 | 6.4 | 6.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人CCL20，未加人 CCL20 的血清作为本底，计算回收率。回收率的范围从 121 % 至 128 %，平均回收率为 115 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-67.43 | 20 | 39.61 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
CCL20，也称为MIP-3α，是一种趋化因子，在树突状细胞运输和T细胞的募集和活化中起重要作用。炎症刺激后，它在单核细胞、T 细胞、内皮细胞、上皮细胞和成纤维细胞中上调。CCL20通过CCR6发出信号，CCR6在记忆T细胞，B细胞和骨髓来源的树突状细胞上表达。