Human Asprosin enzyme-linked immunoassay kit
Catalog# EH0032
Human Asprosin enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
4.69~300 ng/ml
General Overview
Product Name
Human Asprosin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.78 ng/ml (50 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0407 | 0.0468 | 0.0438 | |
| 4.69 | 0.1037 | 0.1032 | 0.1035 | 0.0597 |
| 9.38 | 0.1406 | 0.1638 | 0.1522 | 0.1085 |
| 18.75 | 0.2698 | 0.2895 | 0.2797 | 0.2359 |
| 37.50 | 0.5256 | 0.5766 | 0.5511 | 0.5074 |
| 75.00 | 1.0950 | 1.1600 | 1.1275 | 1.0838 |
| 150.00 | 2.3230 | 2.0280 | 2.1755 | 2.1318 |
| 300.00 | 3.6020 | 3.3780 | 3.4900 | 3.4463 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 6.4 | 29.6 | 112.8 | 5.9 | 31.2 | 118.1 |
| Standard Deviation | 0.3 | 1.2 | 5.9 | 0.3 | 1.3 | 5.4 |
| Coefficient of Variation (%) | 4.0 | 4.0 | 5.2 | 5.1 | 4.2 | 4.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.35-128.74 | 100 | 34.32 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
90min
Standard Curve Gradient
7点
Incubation Count
2
Dosing Volume
50 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
白脂素 (Asprosin)是2016 年由 Romere 等研究发现的一种新型脂肪细胞因子。 其主要由白色脂肪细胞产生并分泌,它一方面能够与肝细胞表面的受体结合,刺激肝细胞葡萄糖输出,直接使血糖升高; 另一方面,能透过血-脑屏障,激活下丘脑中枢摄食相关的刺鼠基因相关蛋白 (AgRP)神经元活性,并抑制厌食相关阿片-促黑素细胞皮质素原 (POMC)神经元活性,促进摄食,间接发挥升血糖作用。