Human IL-8/CXCL8 enzyme-linked immunoassay kit
Catalog# EH0014
Human IL-8/CXCL8 enzyme-linked immunoassay kit
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ELISA
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Human
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4.12~3000 pg/ml
General Overview
Product Name
Human IL-8/CXCL8 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.91 pg/ml (50 μl) ； 2.51 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0712 | 0.0746 | 0.0729 | |
| 4.12 | 0.1577 | 0.1740 | 0.1659 | 0.0930 |
| 12.35 | 0.2702 | 0.2774 | 0.2738 | 0.2009 |
| 37.04 | 0.5827 | 0.6065 | 0.5946 | 0.5217 |
| 111.11 | 1.3060 | 1.3280 | 1.3170 | 1.2441 |
| 333.33 | 2.6770 | 2.6810 | 2.6790 | 2.6061 |
| 1000.00 | 3.8962 | 3.9586 | 3.9274 | 3.8545 |
| 3000.00 | 4.0852 | 4.2235 | 4.1544 | 4.0815 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 59.4 | 303.5 | 910.1 | 57.1 | 304.5 | 902.4 |
| Standard Deviation | 0.5 | 5.8 | 23.9 | 1.2 | 9.8 | 33.2 |
| Coefficient of Variation (%) | 2.9 | 5.6 | 4.7 | 3.5 | 4.3 | 5.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-8，未加人 IL-8 的血清作为本底，计算回收率。回收率的范围从 88 % 至 112 %，平均回收率为 97 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 113.36- 1751.50 | 100 | 741.07 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 3 publications below |
Application in Publications
| Human | See 3 publications below |
Target
UNIPROT
Function
Citation
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IL-8/CXCR2 mediates resistance to apatinib through PI3K/AKT and vasculogenic mimicry in gastric cancer cells
Author: Yan-Wen Fan, Ya-Xian Liu, Ning-Jing Jiang, Si-Tong Qian, Xin-En Huang, Jia-Qi Wu, Ming-Fang He
PMID: 41101042
Journal: Tissue & Cell
Application: ELISA
Reactivity: Human
Publish date: 2025 Oct
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Citation
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Investigating the Role of Quercetin, an Active Ingredient in Bazhen Decoction, in Targeting CXCL8 to Inhibit Macrophage M2 Polarization and Reshape the Immunological Microenvironment of Colorectal Cancer
Author: Wenwu Wang, Fangfeng Lin, Shuping Shi, Yunqiu Yu, Mengyan Lin, Wenliang Lian, Biyin Chen, Xiaoyan Qi
PMID: 39821540
Journal: Chemical Biology & Drug Design
Application: ELISA
Reactivity: Human
Publish date: 2025 Jan
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Citation
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Investigating the Role of Quercetin, an Active Ingredient in Bazhen Decoction, in Targeting CXCL8 to Inhibit Macrophage M2 Polarization and Reshape the Immunological Microenvironment of Colorectal Cancer
Author:
PMID: 10.1111/cbdd.70047
Journal: Chemical Biology & Drug Design
Application: ELISA
Reactivity: Human
Publish date: 2025 Jan
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Citation