Human TNF-α enzyme-linked immunoassay kit
Catalog# EH0002
Human TNF-α enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
General Overview
Product Name
Human TNF-α enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.21 pg/ml (50 μl) ；0.26 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0390 | 0.0402 | 0.0396 | |
| 1.37 | 0.0588 | 0.0628 | 0.0608 | 0.0212 |
| 4.12 | 0.0819 | 0.0885 | 0.0852 | 0.0456 |
| 12.35 | 0.1693 | 0.1712 | 0.1703 | 0.1307 |
| 37.04 | 0.3876 | 0.3956 | 0.3916 | 0.3520 |
| 111.11 | 0.9071 | 0.9969 | 0.9520 | 0.9124 |
| 333.33 | 2.4084 | 2.3694 | 2.3889 | 2.3493 |
| 1000.00 | 4.3651 | 4.1387 | 4.2519 | 4.2123 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 13 | 74.8 | 215.6 | 15.3 | 73.9 | 323.3 |
| Standard Deviation | 0.5 | 3.1 | 7.3 | 0.6 | 2.8 | 12.9 |
| Coefficient of Variation (%) | 3.7 | 4.1 | 3.4 | 4 | 3.8 | 4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人TNF-α，未加人 TNF-α 的血清作为本底，计算回收率。
回收率的范围从 95 % 至 105 %，平均回收率为 99 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.63-3.85 | 100 | 2.08 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 3 publications below |
Application in Publications
| Human | See 3 publications below |
Target
UNIPROT
Function
Citation
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Development and Comprehensive Evaluation of Scarless Circularization Systems for Circular RNA Therapeutics
Author: Linfeng Chen, Lianhao Song, Jiaqi Yang, Tong Li, Rong Ju, Caijun Sun, Zhi Xie
PMID: 40606646
Journal: Molecular Therapy-Nucleic Acids
Application: ELISA
Reactivity: Human
Publish date: 2025 Jun
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Citation
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang
PMID: 39735335
Journal: Bioactive Materials
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec
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Citation
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The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance
Author: Yinfeng Xu,et al
PMID: 39689715
Journal: Immunity
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec
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Citation