Human IL-10 enzyme-linked immunoassay kit
Catalog# EH0009
Human IL-10 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
General Overview
Product Name
Human IL-10 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.89 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0268 | 0.0259 | 0.0264 | |
| 15.63 | 0.0433 | 0.0397 | 0.0415 | 0.0152 |
| 31.25 | 0.0795 | 0.0831 | 0.0813 | 0.0550 |
| 62.50 | 0.1977 | 0.1791 | 0.1884 | 0.1621 |
| 125.00 | 0.4669 | 0.4558 | 0.4614 | 0.4350 |
| 250.00 | 1.1354 | 1.2026 | 1.1690 | 1.1427 |
| 500.00 | 2.7050 | 2.7878 | 2.7464 | 2.7201 |
| 1000.00 | 3.5502 | 3.5608 | 3.5555 | 3.5292 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 18.9 | 122.4 | 353.8 | 20.7 | 107 | 372 |
| Standard Deviation | 1.1 | 7.4 | 24.2 | 1.2 | 6.6 | 16.6 |
| Coefficient of Variation (%) | 5.8 | 6 | 6.8 | 6.2 | 6.1 | 4.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-10，未加人 IL-10 的血清作为本底，计算回收率。
回收率的范围从 91 % 至 119 %，平均回收率为 105 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.93-10.70 | 100 | 3.26 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
3
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 4 publications below |
Application in Publications
| Human | See 4 publications below |
Target
UNIPROT
Function
Citation
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MIR4435–2HG promotes breast cancer evolution through miR-205-5p/UBE2N axis and exosome-mediated macrophage M2-like polarization
Author: Rui Zhang, Ziqi Meng, Xuwei Wu, Zhengri Piao, Tiefeng Jin, Meihua Zhang
PMID: 10.1016/j.cellsig.2025.112287
Journal: Cellular Signalling
Application: ELISA
Reactivity: Human
Publish date: 2025 Nov
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Citation
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Multi-omics insights of immune cells in the risk and prognosis of idiopathic membranous nephropathy
Author: Song Xiaoyi, Zhu Wen, Li Yang, Li Zhanyu, Cao Wanwei, Lu Jieyu, Pan Wanping, Wei Jingyue, Li Man
PMID: 40783650
Journal: Communications Biology
Application: ELISA
Reactivity: Human
Publish date: 2025 Aug
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Citation
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Indoleamine 2,3-dioxygenase 1 alters the proportions of B cell subpopulations in the microenvironment of acute myeloid leukemia
Author: Yao Yu, Liu Yu-ying, Li Jian-feng, Chen Yun-shuo, Shi Lei, Shen Yang, Yang Li-li, Yang Qing
PMID: 40234305
Journal: Molecular Biomedicine
Application: ELISA
Reactivity: Human
Publish date: 2025 Apr
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Citation
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang
PMID: 39735335
Journal: Bioactive Materials
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec
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Citation