Human MMP-13 enzyme-linked immunoassay kit
Catalog# EH0135
Human MMP-13 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
78.13~5000 pg/ml
General Overview
Product Name
Human MMP-13 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
5.09 pg/ml (50 μl)；10.80 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0792 | 0.0843 | 0.0818 | |
| 78.13 | 0.1480 | 0.1524 | 0.1502 | 0.0685 |
| 156.25 | 0.2363 | 0.2171 | 0.2267 | 0.1450 |
| 312.50 | 0.3585 | 0.3677 | 0.3631 | 0.2814 |
| 625.00 | 0.6069 | 0.6087 | 0.6078 | 0.5261 |
| 1250.00 | 1.1020 | 1.0940 | 1.0980 | 1.0163 |
| 2500.00 | 1.8900 | 1.9660 | 1.9280 | 1.8463 |
| 5000.00 | 3.3930 | 3.3560 | 3.3745 | 3.2928 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 56.9 | 372.0 | 1283.4 | 77.7 | 432.4 | 1499.5 |
| Standard Deviation | 3.3 | 22.3 | 74.8 | 2.8 | 13.2 | 38.0 |
| Coefficient of Variation (%) | 5.8 | 6.0 | 5.8 | 3.6 | 3.1 | 2.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 MMP-13，未加人 MMP-13 的血清作为本底，计算回收率。回收率的范围从 92 % 至 117 %，平均回收率为 106 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.75-44.20 | 100 | 21.14 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
MMP13（基质金属肽酶13）参与组织重塑，炎症，细胞因子分泌和激活。它在急性肺损伤，病毒感染和慢性阻塞性肺部疾病中也起着重要作用。 MMP13中的突变导致密苏里州类型（SEMDMO）的脊椎皮phyphyseal发育不良。