Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)
Catalog# EHY0196
Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
1.37~1000 ng/ml
General Overview
Product Name
Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.32 ng/ml（10 μl）
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0114 | 0.0110 | 0.0112 | |
| 1.37 | 0.0200 | 0.0207 | 0.0204 | 0.0092 |
| 4.12 | 0.0413 | 0.0449 | 0.0431 | 0.0319 |
| 12.35 | 0.1168 | 0.1128 | 0.1148 | 0.1036 |
| 37.04 | 0.3379 | 0.3194 | 0.3287 | 0.3175 |
| 111.11 | 0.9453 | 0.9712 | 0.9583 | 0.9471 |
| 333.33 | 2.3420 | 2.4280 | 2.3850 | 2.3738 |
| 1000.00 | 3.9570 | 3.9835 | 3.9703 | 3.9591 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 22.4 | 108.9 | 363.2 | 19.3 | 98.4 | 338.4 |
| Standard Deviation | 1.1 | 5.2 | 17.4 | 0.4 | 2.2 | 8.7 |
| Coefficient of Variation (%) | 5.0 | 4.7 | 4.8 | 1.9 | 2.2 | 2.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Antithrombin III/ATIII，未加人 Antithrombin III/ATIII 的血清作为本底，计算回收率。回收率的范围从 83 % 至 117 %，平均回收率为 101%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 169.32-423.79 | 100 | 306.49 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
蛇苋C1，也称为抗凝血酶III，是主要的血浆蛇苋，抑制凝血酶和Xa因子等血液凝固蛋白酶。