Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
Catalog# EH0153
Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
40.96~10000 pg/ml
General Overview
Product Name
Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.02 pg/ml (50 μl)；4.22 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0068 | 0.0081 | 0.0075 | |
| 40.96 | 0.0280 | 0.0282 | 0.0281 | 0.0207 |
| 102.40 | 0.0630 | 0.0590 | 0.0610 | 0.0536 |
| 256.00 | 0.1461 | 0.1605 | 0.1533 | 0.1459 |
| 640.00 | 0.4384 | 0.4124 | 0.4254 | 0.4180 |
| 1600.00 | 1.1440 | 1.1410 | 1.1425 | 1.1351 |
| 4000.00 | 2.9610 | 2.8740 | 2.9175 | 2.9101 |
| 10000.00 | 4.0079 | 4.1190 | 4.0635 | 4.0560 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 218.3 | 1133.2 | 3538.1 | 221.8 | 1122.4 | 3350.1 |
| Standard Deviation | 6.8 | 40.5 | 130.7 | 6.3 | 60.8 | 184.5 |
| Coefficient of Variation (%) | 3.1 | 3.6 | 3.7 | 2.8 | 5.4 | 5.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Oxytocin-neurophysin 1/OXT，未加人 Oxytocin-neurophysin 1/OXT 的血清作为本底，计算回收率。回收率的范围从 95 % 至 119 %，平均回收率为 103%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 23.53-1127.81 | 100 | 175.25 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
催产素是一种垂体后叶激素，在下丘脑中与其载体蛋白神经physin I一起合成为无活性的前体。它与神经physin一起包装成神经分泌囊泡，并轴突运输到神经垂体的神经末梢， 它被储存或分泌到血液中。