Human IL-1β enzyme-linked immunoassay kit
Catalog# EH0006
Human IL-1β enzyme-linked immunoassay kit
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ELISA
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Human
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3.91~250 pg/ml
General Overview
Product Name
Human IL-1β enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.18 pg/ml (10 μl)；0.09 pg/ml (50 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0296 | 0.0307 | 0.0302 | |
| 3.91 | 0.1330 | 0.1232 | 0.1281 | 0.0980 |
| 7.81 | 0.2132 | 0.2122 | 0.2127 | 0.1826 |
| 15.63 | 0.3820 | 0.3760 | 0.3790 | 0.3489 |
| 31.25 | 0.7406 | 0.6980 | 0.7193 | 0.6892 |
| 62.50 | 1.3710 | 1.2880 | 1.3295 | 1.2994 |
| 125.00 | 2.4490 | 2.3060 | 2.3775 | 2.3474 |
| 250.00 | 3.7620 | 3.6230 | 3.6925 | 3.6624 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 6.0 | 29.6 | 93.7 | 7.2 | 33.6 | 104.4 |
| Standard Deviation | 0.3 | 1.3 | 4.1 | 0.3 | 1.0 | 2.0 |
| Coefficient of Variation (%) | 4.8 | 4.5 | 4.4 | 4.1 | 2.9 | 1.9 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-1β，未加人 IL-1β 的血清作为本底，计算回收率。
回收率的范围从 85 % 至 120 %，平均回收率为 95 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.56-60.87 | 100 | 15.59 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。
n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 1 publications below |
Application in Publications
| Human | See 1 publications below |
Target
UNIPROT
Function
Citation
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Author: Sheng Ye, Quanle Cao, Panxianzhi Ni, Shuting Xiong, Meng Zhong, Tun Yuan, Jing Shan, Jie Liang, Yujiang Fan, Xingdong Zhang
PMID: 39735335
Journal: Bioactive Materials
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec
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Citation