Human CD73 enzyme-linked immunoassay kit
Catalog# EH0093
Human CD73 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
6.86~5000 pg/ml
General Overview
Product Name
Human CD73 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.14 pg/ml (50 μl)；7.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0143 | 0.0140 | 0.0142 | |
| 6.86 | 0.0225 | 0.0222 | 0.0224 | 0.0082 |
| 20.58 | 0.0441 | 0.0415 | 0.0428 | 0.0287 |
| 61.73 | 0.1086 | 0.1172 | 0.1129 | 0.0988 |
| 185.19 | 0.2823 | 0.3088 | 0.2956 | 0.2814 |
| 555.56 | 0.8374 | 0.8978 | 0.8676 | 0.8535 |
| 1666.67 | 2.0810 | 2.2240 | 2.1525 | 2.1384 |
| 5000.00 | 4.0349 | 4.1717 | 4.1033 | 4.0892 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 100.9 | 489.1 | 1584.7 | 97.4 | 462.3 | 1439.6 |
| Standard Deviation | 3.4 | 20.3 | 99.2 | 4.6 | 18.9 | 95.5 |
| Coefficient of Variation (%) | 3.3 | 4.2 | 6.3 | 4.7 | 4.1 | 6.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CD73，未加人 CD73 的血清作为本底，计算回收率。回收率的范围从 87 % 至 124 %，平均回收率为 109 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 373.94-3453.80 | 100 | 1968.34 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
CD73 是一种外外-5'-核苷酸酶，是一种外酶，通过糖基磷脂酰肌醇锚附着在细胞膜上。该酶由大多数细胞类型表达。CD73 的 5'-核苷酸酶活性将细胞外核苷-5'-单磷酸转化为核苷。CD73 是负责产生细胞外腺苷的几种酶之一，腺苷是一种参与炎症和组织损伤反应的信号分子。