Human ACE2 enzyme-linked immunoassay kit
Catalog# EH0224
Human ACE2 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human ACE2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.38 pg/ml (50 μl)；34.73 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0210 | 0.0218 | 0.0214 | |
| 13.72 | 0.0388 | 0.0313 | 0.0351 | 0.0137 |
| 41.15 | 0.0556 | 0.0485 | 0.0521 | 0.0307 |
| 123.46 | 0.1085 | 0.1030 | 0.1058 | 0.0844 |
| 370.37 | 0.2724 | 0.2717 | 0.2721 | 0.2507 |
| 1111.11 | 0.7754 | 0.7572 | 0.7663 | 0.7449 |
| 3333.33 | 1.8560 | 1.8550 | 1.8555 | 1.8341 |
| 10000.00 | 3.5960 | 3.6320 | 3.6140 | 3.5926 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 150.6 | 785.4 | 2461.7 | 149.6 | 799.6 | 2674.8 |
| Standard Deviation | 7.0 | 21.8 | 103.1 | 7.3 | 14.8 | 147.9 |
| Coefficient of Variation (%) | 4.7 | 2.8 | 4.2 | 4.9 | 1.9 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ACE2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 124% with an overall mean recovery of 83%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.34-296.61 | 100 | 139.11 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ACE2 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃