Human IL1RL1/DER4/ST2 enzyme-linked immunoassay kit
Catalog# EH0235
Human IL1RL1/DER4/ST2 enzyme-linked immunoassay kit
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ELISA
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Human
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13.72~10000 pg/ml
Overview
Product Name
Human IL1RL1/DER4/ST2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.59 pg/ml (50 μl)；8.53 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0084 | 0.0073 | 0.0079 | |
| 13.72 | 0.0170 | 0.0168 | 0.0169 | 0.0091 |
| 41.15 | 0.0341 | 0.0368 | 0.0355 | 0.0276 |
| 123.46 | 0.0847 | 0.0891 | 0.0869 | 0.0791 |
| 370.37 | 0.2346 | 0.2441 | 0.2394 | 0.2315 |
| 1111.11 | 0.5879 | 0.5783 | 0.5831 | 0.5753 |
| 3333.33 | 1.5660 | 1.5200 | 1.5430 | 1.5352 |
| 10000.00 | 3.1700 | 3.1730 | 3.1715 | 3.1637 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 211.53 | 980.92 | 2967.31 | 245.35 | 1173.26 | 3093.95 |
| Standard Deviation | 9.43 | 46.15 | 168.39 | 17.87 | 72.26 | 172.49 |
| Coefficient of Variation (%) | 4.5 | 4.7 | 5.7 | 7.3 | 6.2 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL1RL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 120% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 17.61-61.57 | 100.0 | 28.48 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL1RL1 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃