Human Angiotensinogen enzyme-linked immunoassay kit
Catalog# EH0239
Human Angiotensinogen enzyme-linked immunoassay kit
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ELISA
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Human
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4.69~300 ng/ml
Overview
Product Name
Human Angiotensinogen enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.25 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0403 | 0.0513 | 0.0458 | |
| 4.69 | 0.0701 | 0.0763 | 0.0732 | 0.0274 |
| 9.38 | 0.1047 | 0.1146 | 0.1097 | 0.0639 |
| 18.75 | 0.1803 | 0.1798 | 0.1801 | 0.1343 |
| 37.50 | 0.3458 | 0.3212 | 0.3335 | 0.2877 |
| 75.00 | 0.6617 | 0.6878 | 0.6748 | 0.6290 |
| 150.00 | 1.3420 | 1.1830 | 1.2625 | 1.2167 |
| 300.00 | 2.5350 | 2.7620 | 2.6485 | 2.6027 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 6.24 | 31.12 | 96.08 | 6.93 | 30.70 | 97.07 |
| Standard Deviation | 0.44 | 1.54 | 7.09 | 0.49 | 1.67 | 3.79 |
| Coefficient of Variation (%) | 7.1 | 5.0 | 7.4 | 7.1 | 5.4 | 3.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Angiotensinogen into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 120% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 29.69-62.05 | 100.0 | 46.34 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Angiotensinogen in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃