Human Complement factor B enzyme-linked immunoassay kit(one step)
Catalog# EHY0238
Human Complement factor B enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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46.88~3000 ng/ml
Overview
Product Name
Human Complement factor B enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.62 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0687 | 0.0675 | 0.0681 | |
| 46.88 | 0.1696 | 0.1820 | 0.1758 | 0.1077 |
| 93.75 | 0.2560 | 0.2624 | 0.2592 | 0.1911 |
| 187.50 | 0.4569 | 0.4582 | 0.4576 | 0.3895 |
| 375.00 | 0.7900 | 0.7890 | 0.7895 | 0.7214 |
| 750.00 | 1.4130 | 1.4390 | 1.4260 | 1.3579 |
| 1500.00 | 2.2060 | 2.2270 | 2.2165 | 2.1484 |
| 3000.00 | 2.8310 | 2.9550 | 2.8930 | 2.8249 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 85.84 | 349.12 | 1022.87 | 86.70 | 347.83 | 981.61 |
| Standard Deviation | 2.58 | 9.23 | 41.12 | 2.40 | 10.45 | 29.08 |
| Coefficient of Variation (%) | 3.0 | 2.6 | 4.0 | 2.8 | 3.0 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human Complement factor B into health Human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 83.49-233.23 | 100 | 151.46 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Complement factor B in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃