Human NKp46/NCR1 enzyme-linked immunoassay kit
Catalog# EH0221
Human NKp46/NCR1 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
1.37~1000 pg/ml
Overview
Product Name
Human NKp46/NCR1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.06 pg/ml (50 μl)；1.09 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0119 | 0.0135 | 0.0127 | |
| 1.37 | 0.0232 | 0.0240 | 0.0236 | 0.0232 |
| 4.12 | 0.0501 | 0.0495 | 0.0498 | 0.0501 |
| 12.35 | 0.1274 | 0.1232 | 0.1253 | 0.1274 |
| 37.04 | 0.3634 | 0.3362 | 0.3498 | 0.3634 |
| 111.11 | 0.9253 | 0.9344 | 0.9298 | 0.9253 |
| 333.33 | 2.2430 | 2.0810 | 2.1620 | 2.2430 |
| 1000.00 | 3.7100 | 3.7730 | 3.7415 | 3.7100 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 30.2 | 162.4 | 401.4 | 22.2 | 134.6 | 462.6 |
| Standard Deviation | 1.1 | 4.7 | 6.9 | 1.3 | 4.8 | 13.7 |
| Coefficient of Variation (%) | 3.7 | 2.9 | 1.7 | 5.6 | 3.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human NKp46/NCR1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 109% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 115.20-331.30 | 100 | 218.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NKp46/NCR1 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃